Coding

Part:BBa_K1783004

Designed by: Juhye Kang, Kara Huie, Iowis Zhu   Group: iGEM15_UMaryland   (2015-08-14)

Epsilon Cyclase

A. thaliana LCYE (lycopene epsilon cyclase) with chloroplast transit peptide removed. Catalyzes the formation of one epsilon ring on lycopene. Does not act on delta-carotene or gamma-carotene.

Epsilon cyclase functions as an intermediate step in the conversion of lycopene to lutein, supplements of which delay the onset and lessen the symptoms of Age-Related Macular Degeneration. In concert with crtE, crtI, and crtB, which redirect the mevalonate pathway to produce lycopene, epsilon and beta cyclases and hydroxylases catalyze a series of four reactions to produce the two hydroxylated rings that differentiate lutein from lycopene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
    Illegal BamHI site found at 1398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
    Illegal AgeI site found at 1030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1105


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Categories
Parameters
None